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4 Restless Leg Syndrome (RLS) A neurological disorder known as restless leg syndrome, or RLS, causes uncomfortable feelings in the legs that are frequently characterized as tingling, hurting, or crawling. These sensations create an irresistible urge to move, especially during periods of rest or inactivity, such as bedtime
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One microgram of total RNA from each sample was reverse transcribed, and the real-time RT-PCR measurements were performed, as described previously [49], using an apparatus Aria MX (Agilent Technologies, Santa Clara, CA, USA) with the corresponding SYBR Green kit, according to the Promega manufacturers recommendations
The GDF-15 RA use as a potential treatment for obesity stems from observations that elevated tumour-secreted GDF-15 is correlated with WL [99]
